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Journal of Biomedicine and Biotechnology
Volume 2011 (2011), Article ID 479135, 15 pages
Research Article

Obscurin Depletion Impairs Organization of Skeletal Muscle in Developing Zebrafish Embryos

1Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, MI 48109, USA
2Cellular and Molecular Biology Graduate Program, University of Michigan, Ann Arbor, MI 48109, USA

Received 2 May 2011; Revised 10 July 2011; Accepted 27 July 2011

Academic Editor: Robert J. Bloch

Copyright © 2011 Maide Ö. Raeker and Mark W. Russell. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Expression of obscurin A during zebrafish development. (A-C) RT-PCR expression of obscurin A (A,B) and -actin (C) at 3 (lane 2), 4.5 (lane 3), 6 (lane 4), 8.5 (lane 5), 10 (lane 6), 11.5 (lane 7), 13 (lane 8), 15 (lane 9), 24 (lane 10), 33 (lane 11), and 72 (lane 12) hpf time points. Both obscurin A primers demonstrate expression of obscurin by the time the first somites start to form (lane 6: 10 hpf). [Lane 13 represents a control with RT but no RNA; Lane 14 represents a control with RNA but no RT). (D) Immunostaining for obscurin A in a 20 hpf embryos demonstrates localization at the newly forming somite boundary (arrowhead). (E) In a 20 hpf embryo, RNA in situ hybridization using an obscurin A riboprobe demonstrates localization (arrowhead) of the obscurin mRNA to the somite boundary early in boundary formation.

  1. Supplementary Material