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Journal of Biomedicine and Biotechnology
Volume 2011, Article ID 486021, 7 pages
Review Article

Thin Filament-Reconstituted Skinned Muscle Fibers for the Study of Muscle Physiology

1Laboratory for Comprehensive Bioimaging, Riken Quantitative Biology Center, OLABB, 6-2-3 Furuedai, Suita, Osaka 565-0874, Japan
2Department of Cell Physiology, The Jikei University School of Medicine, Tokyo 105-8461, Japan

Received 21 July 2011; Accepted 12 August 2011

Academic Editor: Henk Granzier

Copyright © 2011 Sayaka Higuchi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


We review the use of thin filament-reconstituted muscle fibers in the study of muscle physiology. Thin filament extraction and reconstitution protocol is a powerful technique to study the role of each component of the thin filament. It is also useful for studying the properties of genetically modified molecules such as actin and tropomyosin. We also review the combination of this protocol with sinusoidal analysis, which will provide a solid technique for determining the effect of regulatory proteins on actomyosin interaction and concomitant cross-bridge kinetics. We suggest that thin filament-reconstituted muscle fibers are an ideal system for studying muscle physiology especially when gene modifications of actin or tropomyosin are involved.