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Journal of Biomedicine and Biotechnology
Volume 2011, Article ID 487329, 9 pages
Research Article

Nutritional Requirements for the Improvement of Growth and Sporulation of Several Strains of Monascus purpureus on Solid State Cultivation

1Department of Marine Biotechnology, Iranian Fisheries Research Organization, No. 297, West Fatemi Avenue, P.O. Box 14155-6116, 1411816616 Tehran, Iran
2Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Science, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia
3Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, Selangor, 43400 Serdang, Malaysia
4Biotechnology Research Center, Malaysian Agricultural Research and Development Institute, P.O. Box 12301, 50774 Kuala Lumpur, Malaysia
5Department of Microbiology, Faculty of Biotechnology and Biomolecular Science, Universiti Putra Malaysia, Selangor, 43400 Serdang, Malaysia

Received 12 July 2011; Revised 15 September 2011; Accepted 16 September 2011

Academic Editor: J. Birchler

Copyright © 2011 Zahra Ajdari et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


This paper describes the nutritional requirements for the improvement of growth and sporulation of several strains of Monascus purpureus on solid state cultivation. The findings revealed that glucose enhanced growth of all M. purpureus strains tested but inhibited the sporulation rate. On the other hand, sucrose induced sporulation but inhibited production of cell mass. A combination of glucose and sucrose greatly enhanced sporulation and cell mass production of M. purpureus. Although growth and sporulation rate were related to the ratio of carbon to nitrogen (C/N ratio), the types and concentrations of carbon and nitrogen sources also greatly influenced the growth kinetics. Among the media tested, Hiroi-PDA medium was the most preferred medium for all M. purpureus strains tested for the enhancement of radial growth rate, sporulation, and cell production. Hence, Hiroi-PDA could be suggested as the generic basal medium for the cultivation of M. purpureus. However, individual medium optimization is required for significant enhancement in growth and sporulation of each strain of M. purpureus.