Localization of Magic-F1 Transgene, Involved in Muscular Hypertrophy, during Early Myogenesis

Figure 2

Expression of Magic-F1 in whole mount embryos. Lateral view of whole-mount in situ hybridization with digoxigenin labeled antisense Magic-F1 (a, b, d, e, f, g, h, and i) and sense Magic-F1 (c) cRNA probes as negative control (ctr-). Specific hybrids are visualized as a purple precipitate in all panels except in (a), 9.5 dpc and (b) 10.5 dpc. (e) Specific Magic-F1 transcripts are detected (f) in the mesodermal cells of the jaw and hyoid bone; in f, tv: telencephalic vesicles, cmt: mesenchymal cephalic tissue, hb: hindbrain, and ba: branchial arches where muscles are localized as well as around the eyes; a faint expression is also revealed in tail (g) and dorsal (h) somites, while a strong signal was selected in somites of fore (f^') and hind (h^') limb buds (i). In embryos hybridized with sense probe (c) background staining has not been detected as well as on 11.5 dpc wild type embryo (wt) hybridized with antisense probes (d).