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Journal of Biomedicine and Biotechnology
Volume 2011, Article ID 530926, 5 pages
Research Article

A Proteome Reference Map of the Causative Agent of Melioidosis Burkholderia pseudomallei

1Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand
2National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathumthani 12120, Thailand
3Department of Biochemistry, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, Thailand

Received 15 May 2011; Revised 6 July 2011; Accepted 23 July 2011

Academic Editor: Don Mark Estes

Copyright © 2011 Patompon Wongtrakoongate et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Burkholderia pseudomallei is the etiologic agent of melioidosis. Using 2DE and MALDI-TOF MS, we report here a proteome reference map constructed from early stationary phase, a bacterial adaptation process. We identified 282 protein spots representing 220 ORFs; many of them have been implicated in bacterial pathogenesis. Up to 20% of identified ORFs belong to post-translational modification and stress responses. The proteome reference map will support future analysis of the bacterial gene and environmental regulation and facilitate comparative proteomics with its sibling species.