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Journal of Biomedicine and Biotechnology
Volume 2011 (2011), Article ID 530926, 5 pages
Research Article

A Proteome Reference Map of the Causative Agent of Melioidosis Burkholderia pseudomallei

1Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand
2National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathumthani 12120, Thailand
3Department of Biochemistry, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, Thailand

Received 15 May 2011; Revised 6 July 2011; Accepted 23 July 2011

Academic Editor: Don Mark Estes

Copyright © 2011 Patompon Wongtrakoongate et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary figure 1: Percentages of identified proteins to predicted ORFs of B. pseudomallei genome according to their biological functions.

Supplementary figure 2: Gene ontology classification of the identified genes derived from the proteome reference map of B. pseudomallei grown under early stationary phase.

Supplementary figure 3: Chromosomal preferential expression in B. pseudomallei proteome reference map. Percentages of identified genes (x-axis) were plotted against gene ontology classification (y-axis) according to their chromosomal localization (chromosome 1 or chromosome 2).

Supplementary figure 4: Biological metabolic pathways extracted from gene expression of B. pseudomallei during early stationary phase of growth. Each gene locus was queried against the KEGG website ( using B. pseudomallei strain K96243 database. Gene symbols highlighted in red indicate RpoS-regulated genes reported in reference [9] in the main text.

Table 1: Identification of proteins expressed in early stationary phase culture of Burkholderia pseudomallei by MALDI-TOF MS.

Table 2: Protein isoforms identified in Burkholderia pseudomallei proteome reference map constructed under early stationary phase.

  1. Supplementary Figure 1
  2. Supplementary Figure 2
  3. Supplementary Figure 3
  4. Supplementary Figure 4
  5. Supplementary Table 1
  6. Supplementary Table 2