Alpha 1,3-Galactosyltransferase Deficiency in Pigs Increases Sialyltransferase Activities That Potentially Raise Non-Gal Xenoantigenicity
Figure 3
Expression of full-length CMAH and variants in control and heterozygote α1,3-galactosyltransferase gene (GGTA1) knockout (GalT KO) liver. (a) Electrophoretic analysis of real-time reverse transcriptase-polymerase chain reaction (RT-PCR) in control and heterozygote GalT KO liver. (b) Quantification of real-time RT-PCR analysis in control and heterozygote GalT KO liver. All RT-PCR reactions were conducted in triplicate and normalized for pig H2A histone family, member Z gene (pH2AFZ). Each of the GalT KO relative values is presented as an n-fold expression difference compared to the control, which was set as 1. ***.
