Cytotoxic Effects of Native and Recombinant Frutalin, a Plant Galactose-Binding Lectin, on HeLa Cervical Cancer Cells

Table 1

Biomolecular properties of frutalin extracted from breadfruit seeds (A. incisa) (n-frutalin) and recombinant frutalin produced in P. pastoris (r-frutalin).

Biomolecular properties	Frutalin source		References
	A. incisa	P. pastoris
Primary structure			[14, 15]
	(Several sequences sharing 94–97% identity)	(Defined amino acid sequence)
Molecular weight (kDa)
Glycosylated forms	15.5	18.7±0.2	[11, 19]
Nonglycosylated forms	12	15.9±0.3
Glycosylation content	3.5	2.8±0.3
Mature structure	Tetramer (49 kDa)	Tetramer (62–64 kDa)	[11, 15]
Hemagglutination activity	Minimal concentration: 3.125 μg/mL	Not detected	[15, 20]
Specificity: Me-α-galactose; (association constant, Ka, M^−1)	1.56×10^4	1.38×10^2	[15]