Use of BODIPY (493/503) to Visualize Intramuscular Lipid Droplets
in Skeletal Muscle
Figure 4
High-magnification image demonstrates the presence of LDs within nonslow muscle fibers in the cross section of the rat TA. (a) Fibers stained with BODIPY (green) demonstrate the appearance of spherical lipid droplets within the muscle fibers. (b) Slow fibers were identified using an antibody specific for the slow form of MHC (red). (c) Nuclei were stained with DAPI (Blue). (d) Merged triple label image of the muscle section demonstrates fibers presenting with substantial BODIPY signal that do not label for slow MHC. All images were taken at 63x, and a scale bar is provided for reference.
