Figure 5: Indirect immunofluorescence of Ts-Cav-1 protein expression in muscle larvae (ML) cultured. Four-micrometre sections from parasites cultured without or with sex steroids (P4, E2 or T4) were treated with anti-Ts-Cav-1 antibody and fluorescein isothiocyanate-conjugated goat anti-mouse antibody and observed under confocal laser microscopy. In all cases of parasite treated there was no changes on Ts-Cav-1 protein expression. Bars = 25 μm.