Research Article
MYC, TP53, and Chromosome 17 Copy-Number Alterations in Multiple Gastric Cancer Cell Lines and in Their Parental Primary Tumors
Table 1
FISH analysis of MYC copy number in gastric cancer cell lines, in their parental primary tumors and in control samples.
| | | Nuclei exhibiting MYC signals, no. (%)a | | | 1 signal | 2 signals | 3 signals | 4 signals | ≥5 signals | HA |
| | ACP02 | Parental primary tumor | 0 (0) | 57 (28.5) | 78 (39) | 57 (28.5) | 8 (4) | 0 (0) | | 6th passage | 1 (0.5) | 40 (20) | 84 (42) | 62 (31) | 13 (6.5) | 0(0) | | 12th passage | 0 (0) | 34 (17) | 88 (44) | 63 (31.5) | 15 (7.5) | 0(0) | | 60th passage | 0 (0) | 6 (3) | 83 (41.5) | 79 (39.5) | 24 (12.5) | 7 (3.5) | | 85th passage | 2 (1) | 8 (4) | 65 (32.5) | 84 (42) | 30 (15) | 11 (5.5) |
| | ACP03 | Parental primary tumor | 0 (0) | 39 (19.5) | 84 (42) | 41 (20.5) | 23 (11.5) | 13 (6.5) | | 6th passage | 0(0) | 33 (16.5) | 89 (44.5) | 40 (20) | 24 (12) | 14 (7) | | 12th passage | 1 (0.5) | 19 (9.5) | 85 (42.5) | 51 (25.5) | 29 (14.5) | 15 (7.5) | | 60th passage | 1 (0.5) | 8 (4) | 71 (35.5) | 63 (31.5) | 39 (19.5) | 18 (9) | | 85th passage | 0 (0) | 6 (3) | 47 (23.5) | 69 (34.5) | 49 (24.5) | 29 (14.5) |
| | AGP01 | Parental primary tumor | 0 (0) | 47 (23.5) | 90 (45) | 37 (18.5) | 17 (8.5) | 9 (4.5) | | 6th passage | 4 (2) | 12 (6) | 69 (34.5) | 59 (29.5) | 36 (18) | 20 (10) | | 12th passage | 1 (0.5) | 13 (6.5) | 70 (35) | 56 (28) | 33 (16.5) | 27 (13.5) | | 60th passage | 0 (0) | 2 (1) | 64 (32) | 63 (31.5) | 41 (20.5) | 30 (15) | | 85th passage | 1 (0.5) | 1 (0.5) | 45 (22.5) | 67 (33.5) | 49 (24.5) | 37 (18.5) |
| | Control | Normal stomach tissue | 4 (2) | 194 (97) | 2 (1) | 0 (0) | 0 (0) | 0 (0) | | Lymphocytes | 1 (0.5) | 199 (99.5) | 0 (0) | 0 (0) | 0 (0) | 0 (0) |
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aThe FISH analysis was performed on 200 nuclei. HA: high amplification.
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