Research Article
MYC, TP53, and Chromosome 17 Copy-Number Alterations in Multiple Gastric Cancer Cell Lines and in Their Parental Primary Tumors
Table 2
FISH analysis of chr17/TP53 copy number in gastric cancer cell lines, in their parental primary tumors and in control samples.
| | | Nuclei exhibiting chr17/TP53 signals, no. (%)a | | | 1/0 signal | 1/1 signals | 2/1 signals | 2/2 signals | 2/3 signals | 3/2 signals | 3/3 signals |
| | ACP02 | Parental primary tumor | 3 (1.5) | 4 (2) | 63 (31.5) | 130 (65) | 0 (0) | 0 (0) | 0 (0) | | 6th passage | 2 (1) | 4 (2) | 62 (31) | 132 (66) | 0 (0) | 0 (0) | 0 (0) | | 12th passage | 0 (0) | 7 (3.5) | 73 (36.5) | 120 (60) | 0 (0) | 0 (0) | 0 (0) | | 60th passage | 1 (0.5) | 10 (5) | 84 (42) | 104 (52) | 1 (0.5) | 0 (0) | 0 (0) | | 85th passage | 2 (1) | 25 (12.5) | 102 (51) | 69 (34.5) | 1 (0.5) | 0 (0) | 1 (0.5) |
| | ACP03 | Parental primary tumor | 2 (1) | 14 (7) | 17 (8.5) | 100 (50) | 0 (0) | 54 (27) | 13 (6.5) | | 6th passage | 3 (1.5) | 15 (7.5) | 14 (7) | 86 (43) | 3 (1.5) | 47 (23.5) | 32 (16) | | 12th passage | 4 (2) | 8 (4) | 9 (4.5) | 75 (37.5) | 4 (2) | 55 (27.5) | 45 (22.5) | | 60th passage | 1 (0.5) | 1 (0.5) | 2 (1) | 21 (10.5) | 2 (1) | 70 (35) | 103 (51.5) | | 85th passage | 3 (1.5) | 0 (0) | 5 (2.5) | 6 (3) | 5 (2.5) | 121 (60.5) | 60 (30) |
| | AGP01 | Parental primary tumor | 6 (3) | 29 (14.5) | 7 (3.5) | 116 (58) | 4 (2) | 12 (6) | 26 (13) | | 6th passage | 5 (2.5) | 12 (6) | 4 (2) | 36 (18) | 0 (0) | 31 (15.5) | 112 (56) | | 12th passage | 0 (0) | 9 (4.5) | 3 (1.5) | 35 (17.5) | 6 (3) | 37 (18.5) | 110 (55) | | 60th passage | 4 (2) | 4 (2) | 0 (0) | 30 (15) | 5 (2.5) | 36 (18) | 121 (60.5) | | 85th passage | 3 (1.5) | 7 (3.5) | 0 (0) | 23 (11.5) | 3 (1.5) | 70 (35) | 94 (47) |
| | Control | Normal stomach tissue | 0 (0) | 4 (2) | 1 (0.5) | 193 (96.5) | 0 (0) | 0 (0) | 2 (1) | | Lymphocytes | 0 (0) | 3 (1.5) | 1 (0.5) | 195 (97.5) | 1 (0.5) | 0 (0) | 0 (0) |
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aThe FISH analysis was performed on 200 nuclei.
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