Research Article

Autologous Peripheral Blood Mononuclear Cell Recognition of Autologous Proliferating Tumor Cells in the Context of a Patient-Specific Vaccine Trial

Figure 1

Peripheral blood lymphocytes were placed in the wells of ELISPOT plates in quadruplicate under three separate conditions: unstimulated (−Stm), stimulated with 3000 IU/mL IL-2, 2 μg/mL PHA, and 5 μg/mL OKT3 (+Stm), or combined with autologous tumor cells at a ratio of 1 : 2 (+ATC). After 48 hours, IFN-γ secretion was determined as per manufacturer’s instructions. Numbers indicate spots counted by automated counter.
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