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Journal of Biomedicine and Biotechnology
Volume 2011, Article ID 636170, 7 pages
Research Article

Enhancement of 2,3-Butanediol Production by Klebsiella oxytoca PTCC 1402

1Department of Microbiology, Faculty of Sciences, Islamic Azad University, Rasht Branch, P.O. Box 41335-3516, Rasht, Iran
2Department of Plant Pathology, Faculty of Agriculture, Islamic Azad University, Rasht Branch, P.O. Box 41335-3516, Rasht, Iran

Received 1 November 2010; Accepted 17 December 2010

Academic Editor: J. Birchler

Copyright © 2011 Maesomeh Anvari and Mohammad Reza Safari Motlagh. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Optimal operating parameters of 2,3-Butanediol production using Klebsiella oxytoca under submerged culture conditions are determined by using Taguchi method. The effect of different factors including medium composition, pH, temperature, mixing intensity, and inoculum size on 2,3-butanediol production was analyzed using the Taguchi method in three levels. Based on these analyses the optimum concentrations of glucose, acetic acid, and succinic acid were found to be 6, 0.5, and 1.0 (% w/v), respectively. Furthermore, optimum values for temperature, inoculum size, pH, and the shaking speed were determined as 37°C, 8 (g/L), 6.1, and 150 rpm, respectively. The optimal combinations of factors obtained from the proposed DOE methodology was further validated by conducting fermentation experiments and the obtained results revealed an enhanced 2,3-Butanediol yield of 44%.