Review Article
Myosin Binding Protein-C: A Regulator of Actomyosin Interaction in Striated Muscle
Table 2
Ligands of the skeletal isoforms of MyBP-C.
| Skeletal MyBP-C | MyBP-C Domain | Ligand | Method | Affinity | Reference |
| C1-C2 | Myosin S2 | Cosedimentation assay, ITC | 2.2 μM | [26, 34] | fast C10 | Myosin filaments | Cosedimentation assay | 0.5 μM | [27] | Full length | F-actin | Cosedimentation | | [36, 50] | Full length | Titin 11-domain super repeat | Dot-blot | | [31, 54] | slow variant 1 C10 + insert | Obscurin Ig2 | Y2H, pull down, overlay | | [13] | slow variant 1 C10 + insert | FHL1 | Y2H, pull down, Co-IP | | [55] |
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Abbreviations used: RLC, regulatory light chain; NMR, nuclear magnetic resonance; ITC, isothermal calorimetry; LMM, light meromyosin; Co-IP, coimmunoprecipitation; Y2H, yeast 2 hybrid; SPR, surface plasmon resonance.
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