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Journal of Biomedicine and Biotechnology
Volume 2011 (2011), Article ID 670589, 6 pages
http://dx.doi.org/10.1155/2011/670589
Research Article

Effect of Antimicrobial Peptide-Amide: Indolicidin on Biological Membranes

1Institute of Biophysics, Biological Research Centre, Hungarian Academy of Sciences, P.O. Box 521, 6701 Szeged, Hungary
2Department of Biotechnology, University of Szeged, Közép fasor 52, 6726 Szeged, Hungary

Received 20 January 2011; Revised 24 March 2011; Accepted 29 April 2011

Academic Editor: Miguel Castanho

Copyright © 2011 Attila Gergely Végh et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Indolicidin, a cationic antimicrobial tridecapeptide amide, is rich in proline and tryptophan residues. Its biological activity is intensively studied, but the details how indolicidin interacts with membranes are not fully understood yet. We report here an in situ atomic force microscopic study describing the effect of indolicidin on an artificial supported planar bilayer membrane of dipalmitoyl phosphatidylcholine (DPPC) and on purple membrane of Halobacterium salinarum. Concentration dependent interaction of the peptide and membranes was found in case of DPPC resulting the destruction of the membrane. Purple membrane was much more resistant against indolicidin, probably due to its high protein content. Indolicidin preferred the border of membrane disks, where the lipids are more accessible. These data suggest that the atomic force microscope is a powerful tool in the study of indolicidin-membrane interaction.