Figure 3: Representative flow cytometric histograms of apoptotic Jurkat T-cells stained with annexin V-FITC and propidium iodide (PI). Jurkat T-cells ( /mL) were left untreated or treated with camptothecin (CPT, 8 μM) at 37°C for 24 h, followed by staining with ANV-FITC and PI. Apoptotic cells were identified as ANV+PI- and live cells as ANV-PI- on an FACScan analyser.