Synergic Effect of Genistein and Daidzein on UVB-Induced DNA Damage: An Effective Photoprotective Combination
Figure 1
Effects of UVB irradiation on DNA damage and cell viability. (a) BJ-5ta cells were grown as described under Section 2, exposed to increasing doses of UVB irradiation (from 5 to 60 mJ/cm2) and harvested 24 h after UVB. The Comet assay (see Section 2) was performed according to the manufacturer’s instructions. The results were quantified using the NIH Image Software. The data are reported as tail moment (TM) and represent the mean ± SD of three independent experiments. **, *** (Ctrl: unirradiated control cells). (b, c) Gadd45 and COX-2 mRNA were measured by real time-PCR in a total RNA preparation from exponentially growing human fibroblasts (BJ-5ta) 24 h after UVB irradiation (from 5 to 60 mJ/cm2). The bars indicate the relative abundance of the mRNA; +1 is the abundance of Gadd45 and COX-2 mRNA in unirradiated cells. All values represent the mean ± SD of triplicate experiments. *, **, ***. (d) The viability of BJ-5ta was measured by Trypan blue 24 h after UVB irradiation at increasing doses. The results are reported as percentage of cell viability and the values represent the mean values ± SD of three independent experiments (Ctrl: unirradiated control cells).
