Synergic Effect of Genistein and Daidzein on UVB-Induced DNA Damage: An Effective Photoprotective Combination
Figure 2
Effects of genistein and daidzein on Gadd45 and COX-2 mRNA levels and on BJ-5ta cell viability. (a, c) mRNA levels of Gadd45 and COX-2 were determined by real-time PCR in a total RNA preparation of BJ-5ta cells treated with various concentrations of genistein (from 10 to 60 μM) (a) or daidzein (from 10 to 60 μM) (c) for 24 h. The bars indicate the relative abundance of the mRNA; +1 is the abundance of Gadd45 and COX-2 mRNA in untreated cells. All values represent the mean ± SD of triplicate experiments. **, *** (Ctrl: untreated control cells). (b, d) Cell viability was measured by Trypan blue in BJ-5ta cells incubated with genistein (from 10 to 60 μM) or daidzein (from 10 to 60 μM) for 24 h. The results are reported as percentage of cell viability, and the values represent the mean values ± SD of three independent experiments (Ctrl: untreated control cells).
