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Journal of Biomedicine and Biotechnology
Volume 2011 (2011), Article ID 702179, 12 pages
http://dx.doi.org/10.1155/2011/702179
Research Article

Production of a Solvent, Detergent, and Thermotolerant Lipase by a Newly Isolated Acinetobacter sp. in Submerged and Solid-State Fermentations

Anahita Khoramnia,1 Afshin Ebrahimpour,1 Boon Kee Beh,1 and Oi Ming Lai1,2

1Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Selangor, 43400 Serdang, Malaysia
2Institute of Bioscience, Universiti Putra Malaysia, Selangor, 43400 Serdang, Malaysia

Received 13 April 2011; Revised 3 July 2011; Accepted 3 July 2011

Academic Editor: Alain Filloux

Copyright © 2011 Anahita Khoramnia et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. J. L. Arpigny and K. E. Jaeger, “Bacterial lipolytic enzymes: classification and properties,” Biochemical Journal, vol. 343, no. 1, pp. 177–183, 1999. View at Publisher · View at Google Scholar · View at Scopus
  2. K. E. Jaeger and T. Eggert, “Lipases for biotechnology,” Current Opinion in Biotechnology, vol. 13, no. 4, pp. 390–397, 2002. View at Publisher · View at Google Scholar · View at Scopus
  3. R. Sharma, S. K. Soni, R. M. Vohra, R. S. Jolly, L. K. Gupta, and J. K. Gupta, “Production of extracellular alkaline lipase from a Bacillus sp. RSJ1 and its application in ester hydrolysis,” Indian Journal of Microbiology, vol. 42, no. 1, pp. 49–54, 2002. View at Google Scholar · View at Scopus
  4. C. Schmidt-Dannert, M. L. Rúa, H. Atomi, and R. D. Schmid, “Thermoalkalophilic lipase of Bacillus thermocatenulatus. I. Molecular cloning, nucleotide sequence, purification and some properties,” Biochimica et Biophysica Acta, vol. 1301, no. 1-2, pp. 105–114, 1996. View at Publisher · View at Google Scholar · View at Scopus
  5. A. Schmid, J. S. Dordick, B. Hauer, A. Kiener, M. Wubbolts, and B. Witholt, “Industrial biocatalysis today and tomorrow,” Nature, vol. 409, no. 6817, pp. 258–268, 2001. View at Publisher · View at Google Scholar · View at Scopus
  6. K. E. Jaeger, B. W. Dijkstra, and M. T. Reetz, “Bacterial biocatalysts: molecular biology, three-dimensional structures, and biotechnological applications of lipases,” Annual Review of Microbiology, vol. 53, pp. 315–351, 1999. View at Publisher · View at Google Scholar · View at Scopus
  7. J. Yang, D. Guo, and Y. Yan, “Cloning, expression and characterization of a novel thermal stable and short-chain alcohol tolerant lipase from Burkholderia cepacia strain G63,” Journal of Molecular Catalysis B, vol. 45, no. 3-4, pp. 91–96, 2007. View at Publisher · View at Google Scholar · View at Scopus
  8. W. C. Nobel, “Hospital epidemiology of Acinetobacter infection,” in The Biology of Acinetobacter, K. Towner, E. Bergogne-Bérézin, and C. A. Fewson, Eds., Plenum, New York, NY, USA, 1991. View at Google Scholar
  9. E. A. Snellman and R. R. Colwell, “Acinetobacter lipases: molecular biology, biochemical properties and biotechnological potential,” Journal of Industrial Microbiology and Biotechnology, vol. 31, no. 9, pp. 391–400, 2004. View at Publisher · View at Google Scholar · View at Scopus
  10. S. Benjamin and A. Pandey, “Coconut cake—A potent substrate for the production of lipase by Candida rugosa in solid-state fermentation,” Acta Biotechnologica, vol. 17, no. 3, pp. 241–251, 1997. View at Publisher · View at Google Scholar · View at Scopus
  11. S. Benjamin and A. Pandey, “Mixed-solid substrate fermentation. A novel process for enhanced lipase production by Candida rugosa,” Acta Biotechnologica, vol. 18, no. 4, pp. 315–324, 1998. View at Publisher · View at Google Scholar · View at Scopus
  12. K. H. Hsu, G. C. Lee, and J. F. Shaw, “Promoter analysis and differential expression of the Candida rugosa lipase gene family in response to culture conditions,” Journal of Agricultural and Food Chemistry, vol. 56, no. 6, pp. 1992–1998, 2008. View at Publisher · View at Google Scholar · View at Scopus
  13. T. Ross, Predictive Food Microbiology Models in the Meat Industry, Meat and livestock Australia, Sydney, Australia, 1999.
  14. C. H. Liu, Y. H. Lin, C. Y. Chen, and J. S. Chang, “Characterization of Burkholderia lipase immobilized on celite carriers,” Journal of the Taiwan Institute of Chemical Engineers, vol. 40, no. 4, pp. 359–363, 2009. View at Publisher · View at Google Scholar · View at Scopus
  15. L. Liu, J. Sun, D. Zhang, G. Du, J. Chen, and W. Xu, “Culture conditions optimization of hyaluronic acid production by Streptococcus zooepidemicus based on radial basis function neural network and quantum-behaved particle swarm optimization algorithm,” Enzyme and Microbial Technology, vol. 44, no. 1, pp. 24–32, 2009. View at Publisher · View at Google Scholar · View at Scopus
  16. P. R. Patnaik, “Synthesizing cellular intelligence and artificial intelligence for bioprocesses,” Biotechnology Advances, vol. 24, no. 2, pp. 129–133, 2006. View at Publisher · View at Google Scholar · View at Scopus
  17. G. Kouker and K. E. Jaeger, “Specific and sensitive plate assay for bacterial lipases,” Applied and Environmental Microbiology, vol. 53, no. 1, pp. 211–213, 1987. View at Google Scholar · View at Scopus
  18. A. Pandey, L. Ashakumary, and P. Selvakumar, “Copra waste—A novel substrate for solid-state fermentation,” Bioresource Technology, vol. 51, no. 2-3, pp. 217–220, 1995. View at Publisher · View at Google Scholar · View at Scopus
  19. K. Adinarayana, T. Prabhakar, V. Srinivasulu, M. Anitha Rao, P. Jhansi Lakshmi, and P. Ellaiah, “Optimization of process parameters for cephalosporin C production under solid state fermentation from Acremonium chrysogenum,” Process Biochemistry, vol. 39, no. 2, pp. 171–177, 2003. View at Publisher · View at Google Scholar · View at Scopus
  20. A. K. Gombert, A. L. Pinto, L. R. Castilho, and D. M. G. Freire, “Lipase production by Penicillium restrictum in solid-state fermentation using babassu oil cake as substrate,” Process Biochemistry, vol. 35, no. 1-2, pp. 85–90, 1999. View at Publisher · View at Google Scholar · View at Scopus
  21. D. Y. Kwon and J. S. Rhee, “A simple and rapid colorimetric method for determination of free fatty acids for lipase assay,” Journal of the American Oil Chemists' Society, vol. 63, no. 1, pp. 89–92, 1986. View at Publisher · View at Google Scholar · View at Scopus
  22. A. Ebrahimpour, R. N. Z. R. A. Rahman, D. H. Ean Ch'ng, M. Basri, and A. B. Salleh, “A modeling study by response surface methodology and artificial neural network on culture parameters optimization for thermostable lipase production from a newly isolated thermophilic Geobacillus sp. strain ARM,” BMC Biotechnology, vol. 8, article 96, 2008. View at Publisher · View at Google Scholar · View at Scopus
  23. D. Bas and I. H. Boyaci, “Modeling and optimization I: usability of response surface methodology,” Journal of Food Engineering, vol. 78, no. 3, pp. 836–845, 2007. View at Publisher · View at Google Scholar · View at Scopus
  24. W. G. Cohran and G. M. Cox, Experimental Design, John Wiley & Sons, New York, NY, USA, 2002.
  25. M. L. M. Fernandes, E. B. Saad, J. A. Meira, L. P. Ramos, D. A. Mitchell, and N. Krieger, “Esterification and transesterification reactions catalysed by addition of fermented solids to organic reaction media,” Journal of Molecular Catalysis B, vol. 44, no. 1, pp. 8–13, 2007. View at Publisher · View at Google Scholar · View at Scopus
  26. V. M. G. Lima, N. Krieger, M. I. M. Sarquis, D. A. Mitchell, L. P. Ramos, and J. D. Fontana, “Effect of nitrogen and carbon sources on lipase production by Penicillium aurantiogriseum,” Food Technology and Biotechnology, vol. 41, no. 2, pp. 105–110, 2003. View at Google Scholar · View at Scopus
  27. A. Khoramnia, O. M. Lai, A. Ebrahimpour, C. J. Tanduba, S. V. Tan, and S. Mukhlis, “Thermostable lipase from a newly isolated staphylococcus xylosus strain; process optimization and characterization using RSM and ANN,” Electronic Journal of Biotechnology, vol. 13, no. 5, 22 pages, 2010. View at Google Scholar
  28. J. R. Dutta, P. K. Dutta, and R. Banerjee, “Optimization of culture parameters for extracellular protease production from a newly isolated Pseudomonas sp. using response surface and artificial neural network models,” Process Biochemistry, vol. 39, no. 12, pp. 2193–2198, 2004. View at Publisher · View at Google Scholar · View at Scopus
  29. R. Gupta, N. Gupta, and P. Rathi, “Bacterial lipases: an overview of production, purification and biochemical properties,” Applied Microbiology and Biotechnology, vol. 64, no. 6, pp. 763–781, 2004. View at Publisher · View at Google Scholar · View at Scopus
  30. C. G. Kumar and H. Takagi, “Microbial alkaline proteases: from a bioindustrial viewpoint,” Biotechnology Advances, vol. 17, no. 7, pp. 561–594, 1999. View at Publisher · View at Google Scholar · View at Scopus
  31. S. J. Chen, C. Y. Cheng, and T. L. Chen, “Production of an alkaline lipase by Acinetobacter radioresistens,” Journal of Fermentation and Bioengineering, vol. 86, no. 3, pp. 308–312, 1998. View at Publisher · View at Google Scholar · View at Scopus
  32. J. C. Mateos Diaz, J. A. Rodríguez, S. Roussos et al., “Lipase from the thermotolerant fungus Rhizopus homothallicus is more thermostable when produced using solid state fermentation than liquid fermentation procedures,” Enzyme and Microbial Technology, vol. 39, no. 5, pp. 1042–1050, 2006. View at Publisher · View at Google Scholar · View at Scopus
  33. C. Y. Li, C. Y. Cheng, and T. L. Chen, “Fed-batch production of lipase by Acinetobacter radioresistens using Tween 80 as the carbon source,” Biochemical Engineering Journal, vol. 19, no. 1, pp. 25–31, 2004. View at Publisher · View at Google Scholar · View at Scopus
  34. E. H. Ahmed, T. Raghavendra, and D. Madamwar, “An alkaline lipase from organic solvent tolerant Acinetobacter sp. EH28: application for ethyl caprylate synthesis,” Bioresource Technology, vol. 101, no. 10, pp. 3628–3634, 2010. View at Publisher · View at Google Scholar · View at Scopus
  35. S. Uttatree, P. Winayanuwattikun, and J. Charoenpanich, “Isolation and characterization of a novel thermophilic-organic solvent stable lipase from Acinetobacter baylyi,” Applied Biochemistry and Biotechnology, vol. 162, no. 5, pp. 1362–1376, 2010. View at Publisher · View at Google Scholar · View at Scopus
  36. H. Ogino, “Organic solvent-stable enzymes,” in Protein Adaptation in Extremophiles, K. S. Thomas and T. Thomas, Eds., Nova Science Publishers, Huntington, NY, USA, 2008. View at Google Scholar
  37. H. Ogino, K. Yasui, T. Shiotani, T. Ishihara, and H. Ishikawa, “Organic solvent-tolerant bacterium which secretes an organic solvent- stable proteolytic enzyme,” Applied and Environmental Microbiology, vol. 61, no. 12, pp. 4258–4262, 1995. View at Google Scholar · View at Scopus
  38. A. Ebrahimpour, R. N. Z. R. A. Rahman, M. Basri, and A. B. Salleh, “High level expression and characterization of a novel thermostable, organic solvent tolerant, 1,3-regioselective lipase from Geobacillus sp. strain ARM,” Bioresource Technology, vol. 102, no. 13, pp. 6972–6981, 2011. View at Publisher · View at Google Scholar
  39. N. R. Kamini and H. Iefuji, “Lipase catalyzed methanolysis of vegetable oils in aqueous medium by cryptococcus spp. S-2,” Process Biochemistry, vol. 37, no. 4, pp. 405–410, 2001. View at Publisher · View at Google Scholar · View at Scopus
  40. N. Nawani, N. S. Dosanjh, and J. Kaur, “A novel thermostable lipase from a thermophilic Bacillus sp.: characterization and esterification studies,” Biotechnology Letters, vol. 20, no. 10, pp. 997–1000, 1998. View at Google Scholar · View at Scopus
  41. C. J. Hun, R. N. Z. A. Rahman, A. B. Salleh, and M. Basri, “A newly isolated organic solvent tolerant Bacillus sphaericus 205y producing organic solvent-stable lipase,” Biochemical Engineering Journal, vol. 15, no. 2, pp. 147–151, 2003. View at Publisher · View at Google Scholar
  42. S. F. Lin, “Production and stabilization of a solvent-tolerant alkaline lipase from Pseudomonas pseudoalcaligenes F-111,” Journal of Fermentation and Bioengineering, vol. 82, no. 5, pp. 448–451, 1996. View at Publisher · View at Google Scholar · View at Scopus
  43. C. Schmidt-Dannert, H. Sztajer, W. Stocklein, U. Menge, and R. D. Schmid, “Screening, purification and properties of a thermophilic lipase from Bacillus thermocatenulatus,” Biochimica et Biophysica Acta, vol. 1214, no. 1, pp. 43–53, 1994. View at Publisher · View at Google Scholar · View at Scopus
  44. A. K. Sharma, R. P. Tiwari, and G. S. Hoondal, “Properties of a thermostable and solvent stable extracellular lipase from a Pseudomonas sp. AG-8,” Journal of Basic Microbiology, vol. 41, no. 6, pp. 363–366, 2001. View at Publisher · View at Google Scholar · View at Scopus
  45. M. A. Eltaweel, R. N. Z. R. A. Rahman, A. B. Salleh, and M. Basri, “An organic solvent-stable lipase from Bacillus sp. strain 42,” Annals of Microbiology, vol. 55, no. 3, pp. 187–192, 2005. View at Google Scholar · View at Scopus
  46. J. B. Kristensen, J. Börjesson, M. H. Bruun, F. Tjerneld, and H. Jørgensen, “Use of surface active additives in enzymatic hydrolysis of wheat straw lignocellulose,” Enzyme and Microbial Technology, vol. 40, no. 4, pp. 888–895, 2007. View at Publisher · View at Google Scholar
  47. L. D. Castro-Ochoa, C. Rodríguez-Gómez, G. Valerio-Alfaro, and R. Oliart Ros, “Screening, purification and characterization of the thermoalkalophilic lipase produced by Bacillus thermoleovorans CCR11,” Enzyme and Microbial Technology, vol. 37, no. 6, pp. 648–654, 2005. View at Publisher · View at Google Scholar · View at Scopus
  48. P. Kanjanavas, S. Khuchareontaworn, P. Khawsak et al., “Purification and characterization of organic solvent and detergent tolerant lipase from thermotolerant Bacillus sp. RN2,” International Journal of Molecular Sciences, vol. 11, no. 10, pp. 3783–3792, 2010. View at Publisher · View at Google Scholar · View at Scopus
  49. M. El Khattabi, P. Van Gelder, W. Bitter, and J. Tommassen, “Role of the calcium ion and the disulfide bond in the Burkholderia glumae lipase,” Journal of Molecular Catalysis B, vol. 22, no. 5-6, pp. 329–338, 2003. View at Publisher · View at Google Scholar
  50. H. Shibata, H. Kato, and J. Oda, “Calcium ion-dependent reactivation of a Pseudomonas lipase by its specific modulating protein, LipB,” Journal of Biochemistry, vol. 123, no. 1, pp. 136–141, 1998. View at Google Scholar · View at Scopus
  51. R. Rosenstein and F. Götz, “Staphylococcal lipases: biochemical and molecular characterization,” Biochimie, vol. 82, no. 11, pp. 1005–1014, 2000. View at Publisher · View at Google Scholar · View at Scopus