Research Article
Production of a Solvent, Detergent, and Thermotolerant Lipase by a Newly Isolated Acinetobacter sp. in Submerged and Solid-State Fermentations
Table 2
Experimental design used in ANN studies by using five independent variables showing observed values of lipase activity in SSF.
| | Run | Temperature (°C) | pH | Moisture content (%) | Time (h) | Olive oil (%) | Lipase activity (U/mg protein) |
| | 1 | 40.9 | 8.3 | 69.0 | 146.4 | 4.5 | 15.0 | | 2 | 40.9 | 6.7 | 91.0 | 146.4 | 4.5 | 17.0 | | 3 | 31.1 | 8.3 | 91.0 | 93.6 | 15.5 | 27.5 | | 4 | 40.9 | 8.3 | 91.0 | 93.6 | 4.5 | 15.5 | | 5 | 40.9 | 8.3 | 69.0 | 93.6 | 15.5 | 17.0 | | 6 | 40.9 | 6.7 | 69.0 | 146.4 | 15.5 | 0 | | 7 | 31.1 | 6.7 | 91.0 | 146.4 | 15.5 | 10.0 | | 8 | 31.1 | 8.3 | 69.0 | 146.4 | 15.5 | 0 | | 9 | 40.9 | 6.7 | 91.0 | 93.6 | 15.5 | 16.0 | | 10 | 31.1 | 8.3 | 91.0 | 146.4 | 4.5 | 27.5 | | 11 | 31.1 | 6.7 | 69.0 | 93.6 | 4.5 | 14.0 | | 12 | 27.0 | 7.5 | 80.0 | 120.0 | 10.0 | 0 | | 13 | 45.0 | 7.5 | 80.0 | 120.0 | 10.0 | 0.5 | | 14 | 36.0 | 6.0 | 80.0 | 120.0 | 10.0 | 8.5 | | 15 | 36.0 | 9.0 | 80.0 | 120.0 | 10.0 | 7.0 | | 16 | 36.0 | 7.5 | 60.0 | 120.0 | 10.0 | 20.0 | | 17 | 36.0 | 7.5 | 100 | 120.0 | 10.0 | 8.5 | | 18 | 36.0 | 7.5 | 80.0 | 72.0 | 10.0 | 11.5 | | 19 | 36.0 | 7.5 | 80.0 | 168.0 | 10.0 | 30.5 | | 20 | 36.0 | 7.5 | 80.0 | 120.0 | 0 | 24.5 | | 21 | 36.0 | 7.5 | 80.0 | 120.0 | 20.0 | 0.5 | | 22 | 36.0 | 7.5 | 80.0 | 120.0 | 10.0 | 9.5 | | 23 | 36.0 | 7.5 | 80.0 | 120.0 | 10.0 | 13.0 | | 24 | 36.0 | 7.5 | 80.0 | 120.0 | 10.0 | 11.5 | | 25 | 36.0 | 7.5 | 80.0 | 120.0 | 10.0 | 10.0 | | 26 | 36.0 | 7.5 | 80.0 | 120.0 | 10.0 | 12.5 |
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