In Vivo Imaging of Particle-Induced Inflammation and Osteolysis in the Calvariae of NF𝜅B/Luciferase Transgenic Mice

Figure 4

(a) The mRNA levels of NFκB (p100/p52), TNF-𝛼, IL-1𝛽, RANKL, and COX-2 in calvarial tissues retrieved on the indicated days and subjected to real-time RT-PCR (𝑛=3-5 per bar, Mean±SD, ^†𝑃<.05, ^††𝑃<.01, ^†††𝑃<.005  ^∗𝑃<.001, ^∗∗𝑃<.0005, ^∗∗∗𝑃<.0001 versus value on day 0). (b) Correlation between total influx and mRNA levels of NFκB (p100/p52), TNF-𝛼, IL-1𝛽, RANKL, and COX-2. The correlation coefficient and 𝑃 values were 𝑟=0.808, 𝑃<.0001 for NFκB (p100/p52); 𝑟=0.842, 𝑃<.0001 for TNF-𝛼; 𝑟=0.855, 𝑃<.0001 for IL-1𝛽; 𝑟=0.694, 𝑃<.001 for RANKL; 𝑟=0.712, 𝑃<.0005 for COX-2.

(a)

(b)