In Vivo Imaging of Particle-Induced Inflammation and Osteolysis in the Calvariae of NF𝜅B/Luciferase Transgenic Mice

Table 1

List of primers used in real-time RT-PCR.

Primer ID	Primers(5^′-3^′)	Amplicon size (bp)	Accession no.
NFκB (p100/p52)-F	GCT GAT GGC ACA GGA CGA GA	103	NM019408.2
NFκB (p100/p52)-R	AGC GTG ATA AAT GAC GTG GGC TA
TNF-𝛼-F	AAG CCT GTA GCC CAC GTC GTA	122	NM013693
TNF-𝛼-R	GGC ACC ACT AGT TGG TTG TCT TTG
IL-1𝛽-F	TCC AGG ATG AGG ACA TGA GCA C	105	NM008361
IL-1𝛽-R	GAA CGT CAC ACA CCA GCA GGT TA
RANKL-F	CAT GTG CCA CTG AGA ACC TTG AA	107	NM011613
RANKL-R	CAG GTC CCA GCG CAA TGT AAC
COX-2-F	GCC AGG CTG AAC TTC GAA ACA	91	NM011198
COX-2-R	GCT CAC GAG GCC ACT GAT ACC TA
GAPDH-F	TGT GTC CGT CGT GGA TCT GA	150	NM001001303
GAPDH-R	TTG CTG TTG AAG TCG CAG GAG

NFκB: nuclear factor-kappa B, TNF: tumor-necrosis factor, IL: interleukin, RANKL: receptor activator of nuclear factor kappa-B ligand, COX: cyclooxygenase, GAPDH: glyceroaldehyde-3-phosphate dehydrogenase, bp: base pair.