The Over-expression of the β2 Catalytic Subunit of the Proteasome Decreases Homologous Recombination and Impairs DNA Double-Strand Break Repair in Human Cells

Table 1

Proteasome activity in cells that over-express the β2 subunit of the proteasome.

	Luminescence (RLU) × 10^4
Number of cells	CONTROL	β2 expressing cells
5,000	48.4±0.1	46.4±0.1
7,500	71.0±0.2	68.7±0.8
10,000	107.1±16.7	94.5±2.0

Different numbers of cells were seeded in a multi-well plate for 24 hours. After, the protease activity were determined according to the protocol recommended by the manufacturer. Luminescence was determined as relative light units (RLU) × 10⁴ using a plate luminometer.
Results are reported as mean of 3 independent experiments ± standard error.