BioMed Research International / 2011 / Article / Fig 2

Methodology Report

Simple and Specific Dual-Wavelength Excitable Dye Staining for Glycoprotein Detection in Polyacrylamide Gels and Its Application in Glycoproteomics

Figure 2

Specificity testing of LRSH glycoprotein stain dye. Two-fold serial increases in the nonglycoprotein (BSA)/glycoprotein (horseradish peroxidase, HRP) ratio, ranging from 1 μg/1 μg to 32 μg/1 μg. M represents standard protein molecular weight markers. (a) UV 305-nm excitation and CCD camera detection in a UV transilluminator. (b) Poststaining of the same gel with Coomassie blue.

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