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Journal of Biomedicine and Biotechnology
Volume 2011 (2011), Article ID 780108, 8 pages
Methodology Report

Simple and Specific Dual-Wavelength Excitable Dye Staining for Glycoprotein Detection in Polyacrylamide Gels and Its Application in Glycoproteomics

1Graduate Institute of Biotechnology, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan
2Department of Beauty Science, Meiho University of Technology, Pingtung 91202, Taiwan
3Mass Solutions Technology Co. Ltd., New Taipei City 22101, Taiwan
4Food Science and Nutrition Department, Meiho University of Science and Technology, Pingtung 91202, Taiwan
5Department of Plant Industry, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan

Received 20 May 2011; Accepted 4 August 2011

Academic Editor: Susan A. Rotenberg

Copyright © 2011 Yu-Hsuan Chiang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

In this Supplemental Material, the UV-vis spectrum of LRSH dye was shown in Figure-S1 and its fluorescent spectra excited at different wavelengths were shown in Figure-S2. The detection limit and specificity comparisons between this dye staining and a commercial ProQ Emerald 300 was shown in Figure-S3 and Figure-S5, respectively. The linearity assays obtained in different gel imagers were shown in Figure-S4. The MS/MS spectrum of the other deglycosylated peptide identified from urine uromodulin was shown in Figure S5 and all uromodulin peptides identified by LRSH staining, in-gel digestion and LC-MS/MS analysis were listed in Table S1.

  1. Supplementary Materials