BioMed Research International / 2011 / Article / Tab 1

Research Article

In Vitro Culture Conditions for Maintaining a Complex Population of Human Gastrointestinal Tract Microbiota

Table 1

Medium components for the optimal growth of intestinal microbiotaa.

Chemical componentConc. (g L−1)Chemical componentConc. (g L−1)
HighLowHighLow

Starch5.00.5FeSO4 7H2O0.0050.005
Pectin (citrus)2.00.5NaCl4.54.5
Guar gum1.00.5KCl4.54.5
Porcine gastric typeIII mucin4.00.5KH2PO40.50.5
Xylan (Oat-spelt)2.00.5MgSO4 7H2O1.251.25
Arabinogalactan (larch wood)2.00.5CaCl2 6H2O0.150.15
Inulin1.01.0NaHCO31.51.5
Casein3.03.0Cysteine0.80.8
Peptone water5.00.5Hemin0.050.05
Tryptone5.00.5Tween 801.01.0
Bile salts no.30.40.4Resazurin
(0.01%)
1 mL1 mL
Yeast extract4.54.5Vitamin solution1 mL1 mL

Vitamin solution (mg L−1)
Menadione1.0Vitamin B120.5
Biotin2.0Thiamine4.0
Pantothenate10.0ρ-Aminobenzoic acid5.0
Nicotinamide5.0

aMedia were adapted and modified from previous studies [18, 19].

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