Review Article
Epithelial Cell Coculture Models for Studying Infectious Diseases: Benefits and Limitations
| | Monoculture | Nonscaffolded coculture | Organotypic coculture |
| Composition | Simple | Moderate, multicellular | Complex 3D, multicellular | Interactivity | Direct response to stimulus | Synergistic/antagonistic/intercellular | Synergistic/antagonistic/intercellul- ar/stratified | In vivo likeness | Least similar | Moderately similar | Best model of in vivo | Technical requirements | Simple culture and media components | Distinct media needs, no scaffolding requirements | Extensive expertise in media, culture and perfusion | Contamination | Low risk | Moderate risk with continuous cell lines | Moderate risk with continuous cell lines | Architecture considerations | Monolayer or suspension | Mixed monolayer, suspension, or combination | Scaffolds, gels, permeable barriers, media perfusion |
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