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Journal of Biomedicine and Biotechnology
Volume 2011, Article ID 860652, 9 pages
Research Article

Combined Effects of Surface Morphology and Mechanical Straining Magnitudes on the Differentiation of Mesenchymal Stem Cells without Using Biochemical Reagents

1R&D Center, Genewel Co., Ltd., Sangdaewon 1-dong, Jungwon-gu, Seongnam, Gyeonggi-do 462-724, Republic of Korea
2R&D Center, Genewel Co., Ltd., Baecto-ri, Hyangnam-eup, Hwaseong, Gyeonggi-do 445-924, Republic of Korea
3Department of Biomedical Engineering, Inje University, Gimhae, Gyeongnam 621-749, Republic of Korea
4Cardiovascular and Metabolic Disease Center, FIRST Research Group, Institute of Aged Life Redesign, Inje University, Gimhae, Gyeongnam 621-749, Republic of Korea

Received 22 August 2010; Revised 19 December 2010; Accepted 11 January 2011

Academic Editor: George E. Plopper

Copyright © 2011 Ji-Yeon Jang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Existing studies examining the control of mesenchymal stem cell (MSC) differentiation into desired cell types have used a variety of biochemical reagents such as growth factors despite possible side effects. Recently, the roles of biomimetic microphysical environments have drawn much attention in this field. We studied MSC differentiation and changes in gene expression in relation to osteoblast-like cell and smooth muscle-like cell type resulting from various microphysical environments, including differing magnitudes of tensile strain and substrate geometries for 8 days. In addition, we also investigated the residual effects of those selected microphysical environment factors on the differentiation by ceasing those factors for 3 days. The results of this study showed the effects of the strain magnitudes and surface geometries. However, the genes which are related to the same cell type showed different responses depending on the changes in strain magnitude and surface geometry. Also, different responses were observed three days after the straining was stopped. These data confirm that controlling microenvironments so that they mimic those in vivo contributes to the differentiation of MSCs into specific cell types. And duration of straining engagement was also found to play important roles along with surface geometry.