Combined Effects of Surface Morphology and Mechanical Straining Magnitudes on the Differentiation of Mesenchymal Stem Cells without Using Biochemical Reagents

<table>Fabrication of microgrooved substrates. (A) Procedure of fabrication of microgrooved substrates: (a) the negative photoresist was spin-coated onto a silicon wafer, exposed to UV, and polymerased selectively through photomask. (b) The unpolymerased photoresist was washed out. (c) A silicon wafer was etched through the process of DRIE. (d) Silicone mixture was poured onto the microgrooved silicon wafer, spin-coated, and cured at 125°C for 20 min. (e) The microgrooved substrate was separated from the silicon wafer. (B) The dimensions of each groove were confirmed to be 20 <i>μ</i>m and 3 <i>μ</i>m for width and depth, respectively. (C) SEM images of microgrooved substrates: (a) top view and (b) cross section. (scale bar = 50 <i>μ</i>m).</table>

BioMed Research International

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Figure 1

Figure 1: Combined Effects of Surface Morphology and Mechanical Straining Magnitudes on the Differentiation of Mesenchymal Stem Cells without Using Biochemical Reagents 

Figure 1 | Combined Effects of Surface Morphology and Mechanical Straining Magnitudes on the Differentiation of Mesenchymal Stem Cells without Using Biochemical Reagents 