Combined Effects of Surface Morphology and Mechanical Straining Magnitudes on the Differentiation of Mesenchymal Stem Cells without Using Biochemical Reagents

<table>Staining of ALP and <i>α</i>-SMA. The expressions of ALP and <i>α</i>-SMA in MSCs on day 5 ((a)–(f)) and day 8 ((g)–(l)) are presented in (A) and (B), respectively. The expression of ALP was substantial on day 5 when 3% of straining was imposed on the flat surface ((A)(b)). The expression of <i>α</i>-SMA on the microgrooved surface was enhanced more than on the flat surface. In particular, the highest expression of <i>α</i>-SMA was observed when MSCs were under 10% strain on the microgrooved surface. (All photographs were taken at same magnification level; scale bar = 100 <i>μ</i>m).</table>

BioMed Research International

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Figure 4: Combined Effects of Surface Morphology and Mechanical Straining Magnitudes on the Differentiation of Mesenchymal Stem Cells without Using Biochemical Reagents 

Figure 4 | Combined Effects of Surface Morphology and Mechanical Straining Magnitudes on the Differentiation of Mesenchymal Stem Cells without Using Biochemical Reagents 