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Journal of Biomedicine and Biotechnology
Volume 2011 (2011), Article ID 872065, 6 pages
Research Article

Efficient Protein Transduction Method Using Cationic Peptides and Lipids

1Department of Research and Development, Japan Biological Informatics Consortium (JBiC), 2-4-32 Aomi, Koto-Ku, Tokyo 135-8073, Japan
2Biomedicinal Information Research Center, National Institute of Advanced Industrial Science and Technology (AIST), 2-4-7 Aomi, Koto-Ku, Tokyo 135-0064, Japan

Received 20 May 2011; Revised 23 July 2011; Accepted 10 August 2011

Academic Editor: Jiing-Kuan Yee

Copyright © 2011 Kei Yamaguchi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Cationic peptides termed protein transduction domains (PTDs) have been shown to cross biological membranes efficiently. However, proteins transduced by PTDs become entrapped within the endosomal vesicles and are not delivered into organelles. We have developed a novel protein delivery system to enhance the proton sponge effect, which results in rupture of the endosomes, by using a mixture of Wr-T transporter peptide and a commercially available cationic lipid reagent. This peptide and cationic lipid reagent mixture efficiently delivers a variety of cargo proteins into living cells by releasing them from the endosomes.