Efficient Protein Transduction Method Using Cationic Peptides and Lipids

Figure 2

Wr-T/cationic lipid reagents mixture-mediated delivery of VENUS proteins into living cells. (a, c) Following protein purification, 3′ His tag-VENUS, 3′GST and FLAG tag-VENUS, 3′mCherry and His tag-VENUS, and 3′mKG and His tag-VENUS were analyzed by SDS-PAGE. VENUS proteins were complexed with Wr-T and cationic lipid reagents and then overlaid onto HeLa cells in a serum-free medium. Cellular localization was monitored at the same exposure time by fluorescence microscopy. (b) 3′His tag-VENUS; (c) 3′GST and FLAG tag-VENUS; (e) 3′mCherry tag-VENUS and 3′mKG tag-VENUS.