Expression of Recombinant Human Coagulation Factor VII by the Lizard Leishmania Expression System

Figure 1

Human coagulation factor VII. FVII is a serine protease of 406 residues. Different domains of FVII are shown. Posttranslational modifications of the FVII molecule include (1) γ-carboxylation of 10 glutamic acid residues in the N-terminal part of the molecule, (2) N-glycosylation of asparagine residues in positions 145 and 322, and (3) O-glycosylation of serine residues 52 and 60. ○, gamma-carboxyglutamic acid; ▵, O-glycosylation sites; ◇, N-glycosylation sites; Arg152 and Ile153, cleavage site generated after activation; ☆, joining of two chains of FVIIa by a single disulfide bond between cys135 and cys262.