Development of a Single-Step Subtraction Method for Eukaryotic 18S and 28S Ribonucleic Acids

Table 2

Capture efficiency of 18S and 28S ribosomal RNA and recovery of E. coli RNA using a single-step capture protocol on a selective solid phase.

	18S captured (%)	28S captured (%)	E. coli recovered (%)
500 ng	87	51	4
1000 ng	51	32	46

Note: The input RNA tested was 500 and 1000 ng of mixed mammalian (98%) and E. coli (2%) RNA. Denaturation was performed at 72°C for 10 minutes and annealing at 50°C for 90 minutes.