Development of a Single-Step Subtraction Method for Eukaryotic 18S and 28S Ribonucleic Acids

Table 3

Capture and recovery efficiencies obtained by real-time RT-PCR. Capture was performed at 50°C using 500 ng of input RNA (92.5% mammalian and 7.5% E. coli RNA).

Annealing time (min)	30	60
18S captured (%)	31	20
28S captured (%)	41	71
E. coli recovered (%)	54	50