Development of a Single-Step Subtraction Method for Eukaryotic 18S and 28S Ribonucleic Acids

Table 4

Comparison of subtraction efficiencies between the single-step and the two-step method using a commercially available enrichment kit (MICROBEnrich, Ambion).

	500 ng input			1000 ng input
	Captured 18S (%)	Captured  28S (%)	Recovered E. coli (%)	Captured 18S (%)	Captured 28S (%)	Recovered E. coli (%)
Commercial method	29	40	63	30	50	100
Proposed method	31	41	54	60	92	36