Immunologic Monitoring of Cellular Responses by Dendritic/Tumor Cell Fusion Vaccines

Table 1

Immunologic monitoring.

Inflammatory skin reaction	DTH
T-cell proliferation	[^3H] thymidine uptake
	CFSE dilution
Cytokine profile	ELISPOT assay
	Secretion of cytokines
	Intracellular cytokines
CTL assays	^51Cr-release assays
	Flow cytometry-based cytotoxicity assays (Caspase-3, Anexin-V)
CTL-associated molecules	Perforin
	Granzyme B
	CD107a and b expression in CD8+ T cells
	CD154 expression in CD4+ T cells
T cell phenotype	Multimer analysis
	TCR analysis
Immune suppression assays	CD25, FOXP3, IL-10, TGF-beta
	DTH; delayed type hypersensitivity
	CFSE; 5-(and-6)-carboxyfluorescein diacetate succinimidyl ester
	TCR; T-cell receptor