Simultaneous Identification and Quantification of Canrenone and 11-α-Hydroxy-Canrenone by LC-MS and HPLC-UVD

Table 1

Parameters of the analytical curves, LOD, and LOQ for canrenone and 11-α-hydroxy-canrenone quantification by HPLC-UV.

Standard	Concentration range (g/L)	Calibration curve^a	Correlation coefficients (𝑟^2)	LOD^b	LOQ^c
Canrenone	0.1~3.0	y = 0.4587 + 1.2879x	0.9910	0.1 mg/L	0.5 mg/L
11-α-hydroxy-canrenone	0.1~3.0	y = 0.3681 + 1.2274x	0.9928	0.12 mg/L	0.67 mg/L

^ay and x stand for logarithmic values of peak area and concentration (g/L), respectively.
^bLimit of detection (S/𝑁=3).
^cLimit of quantification (S/𝑁=10).