Simultaneous Identification and Quantification of Canrenone and 11-α-Hydroxy-Canrenone by LC-MS and HPLC-UVD

Table 3

Repeatabilities and intermediate precisions of HPLC-UV method for canrenone and 11-α-hydroxy-canrenone.

	Repeatability^e (𝑛=5)				Intermediate precision^f (𝑛=5)
Samples	Content (g/L)		RSD%		Content (g/L)		RSD%
	a	b	a	b	a	b	a	b
1	1.3813	1.8343			2.2379	2.2127
2	1.3726	1.8387			2.2267	2.1567
3	1.3792	1.8814	0.99	1.03	2.2140	2.1479	1.37	1.21
4	1.4029	1.8471			2.2725	2.1704
5	1.4011	1.8609			2.2086	2.1914

^aCanrenone.
^b11-α-hydroxy-canrenone.
^eFive samples was analyzed, and contents of each solution were used to access repeatability.
^fThe mixed standard solutions with the same concentration were analyzed, and their contents were used to access intermediate precision.