Research Article
BAC Modification through Serial or Simultaneous Use of CRE/Lox Technology
Table 2
Variant specificity.
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The percentage of clones of each type product (inversion, deletion, or WT) present in a PCR band is indicated Lox spacer pairs. Inversion indicates a reaction involving the LoxP site in the pBelo BAC backbone (inverted spacer repeat) while deletions indicate a reaction between the indicated Lox pair (direct spacer repeats). Percentages are based on positives out of 144 samples. |