Construction and Characterization of an Infectious Murine Gammaherpesivrus-68 Bacterial Artificial Chromosome

Figure 2

pMHV-68(BAC)v and MHV-68(loxP) displayed similar growth curves to wild-type MHV-68 in vitro. BHK-21 cells were infected with each virus at an MOI of 0.05. The viral titers in cells and the supernatants harvested at various times after infection were determined by plaque assays.