Construction of Papaya Male and Female BAC Libraries and Application in Physical Mapping of the Sex Chromosomes
SunUp female BAC library and characterization. (a) NotI digests of BAC DNA isolated from 42 random BAC clones from the SunUp BAC library. The vector contains two NotI sites that flank the EcoRI cloning sites. The first and last lanes contain the DNA marker Lambda Ladder PFG marker (New England Biolabs, Beverly, MA, USA) and the middle lane contains the DNA marker PFG midrange I (New England Biolabs, Beverly, MA, USA). (b) Insert size distribution for Sunup female ranged from 85–125 kb with an average insert size of 104 kb.