Novel Image Analysis Approach Quantifies Morphological Characteristics of 3D Breast Culture Acini with Varying Metastatic Potentials
Figure 5
Features capturing the level of cell polarity based on the basal membrane protein integrin 6 density. (a) shows the internal integrin 6 density that is computed as illustrated in (e) where the amount of integrin 6 localized inside the acini is divided to the total amount of integrin 6, (b) shows the cumulative spatial distributions of integrin 6 that is computed as illustrated in (g) where the amount of integrin 6 localized within the concentric circles are divided by the total amount of integrin 6 within the acini, (c) shows the continuity of the integrin 6 along the basal membrane that is measured as illustrated in (f) where the number of times that rays initiate from the centroid oriented at from 1 to 360 degrees intersected with the green fluorophores along the basal membrane is counted and normalized between 0 and 1, and (d) shows the ratio between the amount of integrin 6 colocalized with integrin 3 and the total integrin 6 expression. Error bars correspond to the standard error of the means. Two-sided -tests were performed and cell lines that exhibit statistically significance () are marked with asterisks (*).
