102036.fig.005a
(a)
102036.fig.005b
(b)
102036.fig.005c
(c)
102036.fig.005d
(d)
102036.fig.005e
(e)
102036.fig.005f
(f)
102036.fig.005g
(g)
Figure 5: Features capturing the level of cell polarity based on the basal membrane protein integrin 𝛼 6 density. (a) shows the internal integrin 𝛼 6 density that is computed as illustrated in (e) where the amount of integrin 𝛼 6 localized inside the acini is divided to the total amount of integrin 𝛼 6, (b) shows the cumulative spatial distributions of integrin 𝛼 6 that is computed as illustrated in (g) where the amount of integrin 𝛼 6 localized within the concentric circles are divided by the total amount of integrin 𝛼 6 within the acini, (c) shows the continuity of the integrin 𝛼 6 along the basal membrane that is measured as illustrated in (f) where the number of times that rays initiate from the centroid oriented at from 1 to 360 degrees intersected with the green fluorophores along the basal membrane is counted and normalized between 0 and 1, and (d) shows the ratio between the amount of integrin 𝛼 6 colocalized with integrin 𝛼 3 and the total integrin 𝛼 6 expression. Error bars correspond to the standard error of the means. Two-sided   𝑡 -tests were performed and cell lines that exhibit statistically significance ( 𝑃 < 0 . 0 5 ) are marked with asterisks (*).