Figure 3: Histology before and after injury. We labeled TA muscles with H&E stains. In uninjured muscle, necrotic fibers identified by pale, disrupted cytoplasmic staining and infiltrated by mononuclear cells appeared in all dysferlinopathic strains except A/J (red arrows). All dysferlin mutants had many CNFs (blue arrow heads) before LSI. At D3 post-LSI, necrotic fibers increased in all mutants, with the most widespread area of necrosis in A/J muscle. Extensive myofiber damage soon after LSI was visible in DMDmdx muscle, but not in control or dysferlinopathic muscle.