Figure 4: Effect of BM-MSCs on vascular calcification after balloon angioplasty and the fate of transferred BM-MSCs. (a) Von Kossa staining was performed to detect vascular calcification in lesions black metallic deposit was obviously visualized in medial layers in MSC group, compared control and injury group. (b) Immunofluorescence staining was performed to determine the expression of BMP-2. Results indicated that upregulated BMP-2 was strongly expressed in the neointimal and medial layers of vascular wall in MSC group, compared Control and Injury groups. (c) Labeled BM-MSCs were homing in the intimal layers at 6 weeks after angioplasty. 𝑛 = 8 per group.