Mechanism of Growth Inhibition of Prostate Cancer Xenografts by Valproic Acid
Table 2
Immunohistochemistry staining scores of various markers in tissue microarrays created from cell lines treated with 0, 0.6, and 1.2 mM of valproic acid for 14 days.
LnCaP (in vitro)
Weighted IHC score
Fold change
P value
VPA
0 mM
0.6 mM
1.2 mM
0.6 mM
1.2 mM
0 versus 0.6
0 versus 1.2
0.6 versus 1.2
p21
50
65
110
▲
1.30
▲
2.20
<0.001
<0.001
<0.001
p27
7
13
17
▲
1.86
▲
2.43
<0.001
<0.001
<0.001
CK18
36
53
115
▲
1.47
▲
3.19
<0.001
<0.001
<0.001
AR
164
150
117
▼
0.91
▼
0.71
<0.001
<0.001
<0.001
Ki67
230
175
135
▼
0.76
▼
0.59
<0.001
<0.001
<0.001
C4-2 (in vitro)
Weighted IHC score
Fold change
P value
VPA
0 mM
0.6 mM
1.2 mM
0.6 mM
1.2 mM
0 versus 0.6
0 versus 1.2
0.6 versus 1.2
p21
85
100
140
▲
1.18
▲
1.65
<0.001
<0.001
<0.001
p27
42
58
73
▲
1.38
▲
1.74
<0.001
<0.001
<0.001
CK18
65
80
105
▲
1.23
▲
1.62
<0.001
<0.001
<0.001
AR
220
200
185
▼
0.91
▼
0.84
<0.001
<0.001
<0.001
Ki67
200
180
100
▼
0.90
▼
0.50
<0.001
<0.001
<0.001
▲: significant increase,∆: nonsignificant increase, ▼: significant decrease, ▽: non significant decrease, ◁▷: no change.
