Protegrin-1 Inhibits Dengue NS2B-NS3 Serine Protease and Viral Replication in MK2 Cells
Figure 5
RNA quantification and the percentage of inhibition after applying different concentrations of PG-1 on MK2 cell lines infected with dengue virus. The standard curve was generated by 10-fold serial dilution of known copies of dengue RNA. Viral RNA was extracted from cellular supernatant and the fragment located at the 5′UTR region of the virus genome was amplified using one-step RT-PCR. Significant reduction (P < 0.001) in viral copy number was observed with increasing PG-1 concentration (a). The highest inhibition percentage was observed with 12.5 μM at 24, 48, and 72 hrs (b). PG-1 significantly (P < 0.01) inhibited DENV-2 replication in a dosedependent manner with the greatest inhibition effects at 24 hrs (two-way ANOVA with Bonferroni posttest, mean ± SD).
