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Journal of Biomedicine and Biotechnology
Volume 2012, Article ID 256178, 5 pages
http://dx.doi.org/10.1155/2012/256178
Research Article

The Indolic Diet-Derivative, 3,3′-Diindolylmethane, Induced Apoptosis in Human Colon Cancer Cells through Upregulation of NDRG1

1Pediatric Gastroenterology and Nutrition Unit, Carmel Medical Center, Haifa 34362, Israel
2B. Rappaport School of Medicine, Technion-Israel Institute of Technology, Haifa 31096, Israel
3Department of Evolutionary and Environmental Biology, University of Haifa, Mount Carmel, Haifa 31905, Israel
4Department of Human Biology, University of Haifa, Mount Carmel, Haifa 31905, Israel
5Department of Molecular Genetics, Carmel Medical Center, Haifa 34362, Israel

Received 31 July 2011; Accepted 29 August 2011

Academic Editor: Masa-Aki Shibata

Copyright © 2012 A. Lerner et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

N-myc downstream regulated gene-1 participates in carcinogenesis, angiogenesis, metastases, and anticancer drug resistance. In the present study, we analyzed the expression pattern of N-myc downstream regulated gene-1 following treatment of human colonic cancer cell lines; HCT-116 (well differentiated with wild-type p53 gene) and Colo-320 (poorly differentiated with mutant p53 gene), with 3,3′-diindolylmethane, a well-established proapoptotic agent product derived from indole-3-carbinol. Treatment of Colo-320 and HCT-116 with 3,3′-diindolylmethane disclosed inhibition of cell viability in a dose-dependent manner, mediated through apoptosis induction. The increased expression of N-myc downstream regulated gene-1 was detected only in poorly differentiated colon cancer cells, Colo-320 cell line. Our results suggest that N-myc downstream regulated gene-1 expression is enhanced by 3,3′-diindolylmethane in poorly differentiated cells and followed by induction of apoptosis. 3,3′-diindolylmethane induced apoptosis may represent a new regulator of N-myc downstream regulated gene-1 in poorly differentiated colonic cancer cells.