Assessment of the Immune Responses Induced in Cattle after Inoculation of a Mycobacterium bovis Strain Deleted in Two mce2 Genes
Figure 1
Construction of the M. bovis Δmce2 mutant strain. (a) An unmarked mutant strain of M. bovis carrying a chromosomal deletion of the region spanning the mce2A-mce2B genes was created by the gene knock-out system described by Parish and Stoker [15]. The mutant strain was designated MbΔmce2. (b) PCR analysis of MbΔmce2 and the wild type strains. PCRs were performed by using primers that hybridize outside the mce2A-mce2B region (lanes 3 and 5) or in sigA (lanes 2 and 4). Chromosomal DNA from the parental strain (lanes 2 and 3) or from the mutant strain (lanes 4 and 5) was used as template in the PCR reactions. Lane 1 is the molecular weight marker (1Kb ladder Promega).
